Supported Liquid-Liquid Extraction

Traditional LLE utilizes large volumes of solvents that are often hazardous from an environmental perspective and the process is tedious and time consuming. During the past decade, this technique has undergone a spectacular transformation in the context of high-throughput screening with the introduction of miniaturized protocols along with revolutionary membrane-based and solid support based technologies. These advances were reviewed by several authors. The simplest technique is the use of the 96-well collection plate format (analogous to the format used in SPE) in conjunction with a liquid handling robotic system; it follows the same principle as bulk scale LLE.

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