Coral Sephadex Packed Plates

Dye – Terminator removal is one of the major cleanup objective to yield a highly purified product prior to conducting a sequencing reaction. OrochemSephadex packed plates provide a very cost effective solution for high performance parallel processing of 96 sequencing reactions by gel filtration.

• OrochemSephadex packed plates are available in 96-wells and 384-wells formats.
• These plates can be used with gel filtration media for high throughput sequencing reaction cleanup.
• They are constructed from rigid polystyrene that can withstand centrifugation.

General Protocol

• Place the Sephadex plate over appropriate wash plates and place it into a centrifuge with correct carrier.
• Centrifuge for 1 minute at 750 ×g to remove residual buffer. Discard the wash plates containing excess water.
• Carefully and rapidly load 50 µl sequencing reaction mixture before the Spheadex gel dries out.
• Centrifuge for 2 minutes at 750 ×g to eliminate big dye terminators, salts, and low molecular weight species.
• Purified DNA of the sequencing reaction is recovered in collection plate