HPLC Column

Orochem Technologies provides a wide range of traditional liquid chromatography columns for analytical, preparative, and simulated moving bed chromatography (SMB) applications. Our HPLC and semi/prep columns are used globally in clinical diagnostics laboratories, industries (like pharmaceutical, biotech, food, nutraceutical), environmental and forensic testing laboratories.
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HPLC Column Selection

Column selection is based primarily on the solubility of the analytes in solvents. HPLC analytes are typically organic molecules that vary in size, polarity, and functionality. These features govern the solubility of the analyte in solvents that range from highly polar to non-polar. Some analytes have ionic functionality. Ionic analytes often require pH or ionic strength adjustments for good chromatography.

Reversed-Phase Chromatography is the branch of HPLC that uses a hydrophobic treated silica as the stationary phase. The hydrophobic treatment is derived from a silane bonding agent. Polar solvents, including water, are typically used as the mobile phase. The analytes are separated based on their retention behavior on the stationary phase. More retentive species elute later from the stationary phase. The mobile phase concentration is adjusted to give the best chromatography.

Hilic/Normal Phase Chromatography is the branch of HPLC that uses a hydrophilic silica surface as the stationary phase. Hilic/ Normal phase chromatography separates analytes based on their affinity for polar stationary phases. The analytes are typically soluble in non-polar solvents. Many normal phases are derived from silane bonding agents. Bear silica is also used in normal phase HPLC. Adjustments in mobile phase concentration are made to give the best chromatography.


Reliasil HPLC columns are designed for high efficiency (high plate count) and general utility. The proprietary silica gel manufacturing process yields a Gaussian particle size distribution, narrow pore-size distribution, and strong resistance to high pH.

Phases: Silica, C1, C4, C8, C18, C30, Nh2, CN, Phenyl, SCX, SAX, Diol, Polyamine, PFP
pH Range: 2-8.5,


Orosil HPLC columns are designed for the separation of polar, semi-polar, and nonpolar compounds at low to medium pH

Phases: C8, C18
pH Range: 1.5-9.0

Orosil Polar C18
Orosil Polar C18 columns are particularly advantageous for difficult separations involving polar and semi-polar compounds.

Phases: C18
pH Range: 1.5-9.0


Matrix C18 uses base-deactivated, ultra-high purity silica gel, densely bonded to produce an inert material ideally suited for the analysis of basic compounds. The resulting hydrophobic surface is also stable over a wide pH range (2-9) to allow for excellent method flexibility.
Matix C18 AQ- Polar analysis in high Aqueous Mobile phases. Interspersed with hydrophilic end-capping group.

Phases: C8, C18, C18AQ, Nh2, Polychrome
pH Range: 2-9.0


Monitor is a top-quality phase, providing excellent stability and reproducibility, and minimal secondary interaction. Monitor is a perfect choice for reversed phase separations requiring thoroughly base-deactivated columns, and excels in challenging pharmaceutical and peptide separations. We developed Monitor expressly for the chromatography of difficult-to-separate samples.

Phases: silica, C8, C18
pH Range: 2-8

  • Application Note
  • Flyer / Documents
Isocratic Separation of Aliphatic Alcohols on an Orosil C18-HC Column Using UV Detection Download (311 KB)
Isocratic Separation of Ten Cannabinoids on Orosil C18-HC, 3 μm Download (311 KB)
Isocratic Separation of Ten Cannabinoids on Gazelle C18, 1.7 μm Download (308 KB)
Analysis of Tocotrienols and Tocopherols on Orosil C18, 5 μm Download (381 KB)
Analysis of Tocotrienols and Tocopherols on Gazelle C18, 1.7 μm Download (375 KB)
Isocratic Analysis of the Alcohols Present in a Generic Hand/Spray Sanitizer Download (468 KB)
Isocratic Analysis of Seven Cannabinoids on Orosil C18-HC Download (261 KB)
Isocratic Analysis of TEN Cannabinoids on Orosil C18-HC Download (337 KB)
Isocratic Analysis of Cannabinoids on Orosil C18-HC Download (267 KB)
Separation of α- and β-Carotenes on C18-HC (High Carbon) Download (310 KB)
Selectivity of Orosil C18-HC Download (254 KB)
Mephedrone Analysis by HPLC Download (882 KB)
Comprehensive Approaches for Cannabis Product Testing Potency and Pesticide Residue Analysis Download (882 KB)
BSA Tryptic Digest analysis-LCRE-C18-18107 Download (227 KB)
Peptide Mixture-LCRE-C18-18109 Download (232 KB)
Intact Proteins-LCRE-C4-18108 Download (314 KB)
Matrix C18-Analgesics Download (139 KB)
Matrix C18-Azithromycin Download (85 KB)
Matrix Polychrome-beta and dexa-methasone Download (52 KB)
Matrix Polychrome-Carboplatin Download (40 KB)
Matrix Polychrome-Decitabine Download (39 KB)
Matrix-Polychrome-(3epi)-25OH VD3 Download (71 KB)
Matrix-Polychrome-Epitestosterone-testosterone Download (49 KB)
Matrix-Polychrome-Epitestosterone-testosterone-LCMSMS Download (70 KB)
Matrix-Polychrome-hydrocortisone-prednisolone Download (118 KB)
Monitor C8-Carvadilol-BP method Download (50 KB)
Monitor C18-Analgesics Download (141 KB)
Orosil C8- carvedilol BP method Download (49 KB)
Orosil C18-Analgesics Download (57 KB)
Orosil C30-Carotenes Download (134 KB)
Orosil C30-Carotenes-2 Download (153 KB)
Orosil ODS-Drug impurities Download (320 KB)
Orosil ODS-Omega-3 Download (127 KB)
Orosil Polar C18-Aromatic Amines Download (105 KB)
Orosil silica-Orange Juice Download (166 KB)
Orosil-C18-Tamoxifen-Pregabalin-Budesonide-Eszopiclone-Eprosartan-Stavudine-Ciprofloxacin-Atracurium Download (190 KB)
Orosil-Phenyl-Hexyl-Vitamin E Download (117 KB)
Presstige-C18-Sun screen reagents Download (65 KB)
Prestige C18-Pesticides Download (150 KB)
Prestige-C18-Mycotoxin Download (261 KB)
Prestige-C18-Neutral Basic Polar Analytes Download (390 KB)
Reliasil Amino-Steviol Glycosides Download (130 KB)
Reliasil C8-carvedilol BP method Download (51 KB)
Reliasil C18-Aromatic Amines Download (105 KB)
Reliasil C18-Xanthines Download (105 KB)
Reliasil Download (202 KB)
Reliasil-Amino-xylose-galactose-glucose-mannose-arabinose Download (65 KB)
Reliasil-C8-carvedilol Download (327 KB)
Reliasil-C18-nicotine Download (100 KB)
Reliasil-C18-peptides Separation Download (318 KB)
Reliasil-ODS1-Flavanoids- Download (153 KB)
Reliasil-phenyl-Tetrahydrocannabinol Download (197 KB)
Oligonucleotides (Oligo2024) LCRE-C8-22001 Download (196 KB)

HPLC Column

HPLC Column

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