General Steps of an SPE protocol:

• Sample Pre-treatment: Dependent on compound of interest, sample matrix, and nature of retention chemistry; involves pH adjustment, centrifugation, filtration, dilution, buffer addition, etc.
• Conditioning: Solvent is passed through the SPE material to wet the bonded functional groups => ensures consistent interaction.
• Equilibration: Sorbent/ phase is treated with a solution that is similar (in polarity, pH, etc.) to the sample matrix => maximizes retention.
• Sample Load: Introduction of the sample = analytes of interest are bound/ extracted onto the phase/sorbent.
• Washing: Selectively remove unwanted interferences co-extracted with the analyte without prematurely eluting analytes of interest.
• Elution: Removing analytes of interest with a solvent that overcomes the primary and secondary retention interactions between sorbent and analytes of interest.
• Evaporation of eluent/ reconstitution with mobile phase (optional).

Strategies in carrying out SPE

There are 3 different elution strategies in SPE. Which one to choose depends on the goal of the extraction.

i. Bind-Elute Strategy

• Most common
• Bind: Analytes bind to tube, unwanted matrix components are washed off
• Elute: Eluant changed to remove analytes from tube
• Analytes are concentrated via evaporation prior to HPLC or GC analysis

ii. Interference Removal Strategy

• Bind all unwanted matrix components and allow analytes to pass through during the sample loading stage
• Like chemical filtration

iii. Fractionation Strategy

• Retain and sequentially elute different classes of compounds by modifying eluant pH or % organic

SPE Products Selection Guide

Stationary Phase Selection Guide

SPE Hardware Selection Guide

SAMPLE PREP

ORPHEUS	CLASSIC SPE CARTRIDGE	1, 3, 6, 12, 20, 60 ML
ORO-SEP	CARTRIDGE WITH FEMALE & MALE LUER TIPS
LARGE RESERVOIR COLUMN (LRC)	LARGE RESERVOIR ON THE TOP & REGULAR 1 OR 3 ML CARTRIDGE WITH SORBENT AT THE BOTTOM	10 ML
OROSCREEN	LARGE SPE CARTRIDGE	8 ML
OMNISORB	96-WELL PLATE	1 ML/WELL
OROCHEM 2000	96-WELL PLATE	2 ML/WELL
SUPERSCREEN DISC	DISC COLUMNS	6 or 8 MM DISC
	96-WELL DISC PLATE	6 MM DISC 1 ML/WELL
OROCHEM 3000	384-WELL DISC PLATE

Features 

Oro-Sep SPE Cartridges

Oro-Sep Cartridges are molded from medical grade virgin polypropylene, fritted with 20 micron PE frits and are compatible with a syringe or a vacuum manifold. The female luer tip inlet of the cartridge allows connection to a syringe tip. The male luer tip outlet connects to a vacuum manifold. Sep-Pak^®cartridges may be substituted with Oro-Sep cartridges. Oro-Sep cartridges packed with reversed phases, normal phases, and ion exchange phases in three different bed sizes, may be utilized for sample preparation and sample concentration in the bio-tech, clinical, environmental, forensic, and pharmaceutical arena.

SuPErscreen Disc - SPE

Orochem Technologies has now developed SuPErScreen, a proprietary high capacity silica particle embedded glass fiber for Disc Solid Phase Extraction. Elimination of sorbent fines and decreased sample elution columns provide cleaner extracts which allows you to bypass evaporation prior to detection.

SuPErScreen Silica impregnated glass fiber discs are manufactured using both long and short glass fibers offering greater porosity of the matrix. With silica particles present as an integral component during manufacturing, uniform distribution of silica is achieved across the thickness of the disc membrane. A typical 8 mm diameter OROCHEM disc in a 3 ml column contains approximately 13.8 mg of silica.

OmniSorb SPE Plates

• Omnisorb 96 well filter plates are ideal for all SPE applications and High Throughput Screening.
• 1ml sample volume per well Robotic and liquid handling compatibility Choice of chemistries.
• Made from solvent resistant, low extractable polypropylene with unique drip directors.

Orochem 2000 Sorbent filled SPE Plate

Features & Benefits

OROCHEM 2000 SPE 96-well Plates are packed with Orpheus silica based sorbents that have broad selectivity and resist occlusion by proteinaceous materials from samples. Non-occlusion of sorbents, combination of size exclusion and adsorption chromatography allows efficient flow characteristics, enhanced detection, and cleaner extracts. The 2.2 mL/well SPE plates manufactured from virgin medical grade polypropylene have the SBS standard footprint. Individually fritted wells eliminate cross-talk; the larger exit diameter of the well enables a faster flow rate. The OROCHEM 2000 Vacuum Manifold is designed to accommodate every filter plate, and comes with inserts to accommodate collection plates of all sizes, including shallow well plates

Applications

Sample preparation by Solid Phase Extraction in drug development, drug analysis, pharmacokinetics, and biotechnological applications.

Orochem SuPErscreen Disc- SPE Plate

Features & Benefits

Orochem SuPErScreen Disc SPE is manufactured using both long and short glass fibers offering greater porosity of the matrix. With silica particles present as an integral component during manufacturing, uniform distribution of silica is achieved across the thickness of the disc membrane. The use of thick glass fibers offers increased contact with the phase. With SuPErScreen disc SPE, an 8mm diameter disc allows 13.8 mg and a 6mm disc in a 96-well format allows 7.6mg /well media loading capacity.

Applications

Sample preparation by Solid Phase Extraction in drug development, drug analysis, pharmacokinetics, and biotechnological applications.

Orochem 3000 384-well Disc SPE Plate

Orochem 3000 SPE plates are available with SuPErScreen glass fiber based SPE discs. The high throughput 384-well SPE plate enables a four-fold higher sample preparation capability. Orochem 384-well SPE plates offer yet another example of our capabilities to customize sample preparation.