HPLC Column

Orochem Technologies provides a wide range of traditional liquid chromatography columns for analytical, preparative, and simulated moving bed chromatography (SMB) applications. Our HPLC and semi/prep columns are used globally in clinical diagnostics laboratories, industries (like pharmaceutical, biotech, food, nutraceutical), environmental and forensic testing laboratories.
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HPLC Column Selection

Column selection is based primarily on the solubility of the analytes in solvents. HPLC analytes are typically organic molecules that vary in size, polarity, and functionality. These features govern the solubility of the analyte in solvents that range from highly polar to non-polar. Some analytes have ionic functionality. Ionic analytes often require pH or ionic strength adjustments for good chromatography.

Reversed-Phase Chromatography is the branch of HPLC that uses a hydrophobic treated silica as the stationary phase. The hydrophobic treatment is derived from a silane bonding agent. Polar solvents, including water, are typically used as the mobile phase. The analytes are separated based on their retention behavior on the stationary phase. More retentive species elute later from the stationary phase. The mobile phase concentration is adjusted to give the best chromatography.

Hilic/Normal Phase Chromatography is the branch of HPLC that uses a hydrophilic silica surface as the stationary phase. Hilic/ Normal phase chromatography separates analytes based on their affinity for polar stationary phases. The analytes are typically soluble in non-polar solvents. Many normal phases are derived from silane bonding agents. Bear silica is also used in normal phase HPLC. Adjustments in mobile phase concentration are made to give the best chromatography.


Reliasil HPLC columns are designed for high efficiency (high plate count) and general utility. The proprietary silica gel manufacturing process yields a Gaussian particle size distribution, narrow pore-size distribution, and strong resistance to high pH.

Phases: Silica, C1, C4, C8, C18, C30, NH2, CN, Phenyl, SCX, SAX, Diol, Polyamine, PFP
pH Range: 2-8.5,


Orosil HPLC columns are designed for the separation of polar, semi-polar, and nonpolar compounds at low to medium pH

Phases: C8, C18
pH Range: 1.5-9.0

Orosil Polar C18
Orosil Polar C18 columns are particularly advantageous for difficult separations involving polar and semi-polar compounds.

Phases: C18
pH Range: 1.5-9.0


Matrix C18 uses base-deactivated, ultra-high purity silica gel, densely bonded to produce an inert material ideally suited for the analysis of basic compounds. The resulting hydrophobic surface is also stable over a wide pH range (2-9) to allow for excellent method flexibility.
Matix C18 AQ- Polar analysis in high Aqueous Mobile phases. Interspersed with hydrophilic end-capping group.

Phases: C8, C18, C18AQ, NH2, Polychrome
pH Range: 2-9.0


Monitor is a top-quality phase, providing excellent stability and reproducibility, and minimal secondary interaction. Monitor is a perfect choice for reversed phase separations requiring thoroughly base-deactivated columns, and excels in challenging pharmaceutical and peptide separations. We developed Monitor expressly for the chromatography of difficult-to-separate samples.

Phases: silica, C8, C18
pH Range: 2-8

Showing 1–12 of 545 results

  • Catalog#
  • Description
Isocratic Separation of Aliphatic Alcohols on an Orosil C18-HC Column Using UV DetectionFile size: 311 KBDownload
Isocratic Separation of Ten Cannabinoids on Orosil C18-HC, 3 μmFile size: 311 KBDownload
Isocratic Separation of Ten Cannabinoids on Gazelle C18, 1.7 μmFile size: 308 KBDownload
Analysis of Tocotrienols and Tocopherols on Orosil C18, 5 μmFile size: 381 KBDownload
Analysis of Tocotrienols and Tocopherols on Gazelle C18, 1.7 μmFile size: 375 KBDownload
Isocratic Analysis of the Alcohols Present in a Generic Hand/Spray SanitizerFile size: 468 KBDownload
Isocratic Analysis of Seven Cannabinoids on Orosil C18-HCFile size: 261 KBDownload
Isocratic Analysis of TEN Cannabinoids on Orosil C18-HCFile size: 337 KBDownload
Isocratic Analysis of Cannabinoids on Orosil C18-HCFile size: 267 KBDownload
Separation of α- and β-Carotenes on C18-HC (High Carbon)File size: 310 KBDownload
Selectivity of Orosil C18-HCFile size: 254 KBDownload
Mephedrone Analysis by HPLC File size: 882KBDownload
Comprehensive Approaches for Cannabis Product Testing Potency and Pesticide Residue AnalysisFile size: 882KBDownload
BSA Tryptic Digest analysis-LCRE-C18-18107File size: 227KBDownload
Peptide Mixture-LCRE-C18-18109File size: 232KBDownload
Intact Proteins-LCRE-C4-18108File size: 314KBDownload
Matrix C18-AnalgesicsFile size: 139KBDownload
Matrix C18-AzithromycinFile size: 85KBDownload
Matrix Polychrome-beta and dexa-methasoneFile size: 52KBDownload
Matrix Polychrome-CarboplatinFile size: 40KBDownload
Matrix Polychrome-DecitabineFile size: 39KBDownload
Matrix-Polychrome-(3epi)-25OH VD3File size: 71KBDownload
Matrix-Polychrome-Epitestosterone-testosteroneFile size: 49KBDownload
Matrix-Polychrome-Epitestosterone-testosterone-LCMSMSFile size: 70KBDownload
Matrix-Polychrome-hydrocortisone-prednisoloneFile size: 118KBDownload
Monitor C8-Carvadilol-BP methodFile size: 50KBDownload
Monitor C18-AnalgesicsFile size: 141KBDownload
Orosil C8- carvedilol BP methodFile size: 49KBDownload
Orosil C18-AnalgesicsFile size: 57KBDownload
Orosil C30-CarotenesFile size: 134KBDownload
Orosil C30-Carotenes-2File size: 153KBDownload
Orosil ODS-Drug impuritiesFile size: 320KBDownload
Orosil ODS-Omega-3File size: 127KBDownload
Orosil Polar C18-Aromatic AminesFile size: 105KBDownload
Orosil silica-Orange JuiceFile size: 166KBDownload
Orosil-C18-Tamoxifen-Pregabalin-Budesonide-Eszopiclone-Eprosartan-Stavudine-Ciprofloxacin-AtracuriumFile size: 190KBDownload
Orosil-Phenyl-Hexyl-Vitamin EFile size: 117KBDownload
Presstige-C18-Sun screen reagentsFile size: 65KBDownload
Prestige C18-PesticidesFile size: 150KBDownload
Prestige-C18-MycotoxinFile size: 261KBDownload
Prestige-C18-Neutral Basic Polar AnalytesFile size: 390KBDownload
Reliasil Amino-Steviol GlycosidesFile size: 130KBDownload
Reliasil C8-carvedilol BP methodFile size: 51KBDownload
Reliasil C18-Aromatic AminesFile size: 105KBDownload
Reliasil C18-XanthinesFile size: 105KBDownload
ReliasilFile size: 202KBDownload
Reliasil-Amino-xylose-galactose-glucose-mannose-arabinoseFile size: 65KBDownload
Reliasil-C8-carvedilolFile size: 327KBDownload
Reliasil-C18-nicotineFile size: 100KBDownload
Reliasil-C18-peptides SeparationFile size: 318KBDownload
Reliasil-ODS1-Flavanoids-File size: 153KBDownload
Reliasil-phenyl-TetrahydrocannabinolFile size: 197KBDownload