HPLC Column

Orochem Technologies provides a wide range of traditional liquid chromatography columns for analytical, preparative, and simulated moving bed chromatography (SMB) applications. Our HPLC and semi/prep columns are used globally in clinical diagnostics laboratories, industries (like pharmaceutical, biotech, food, nutraceutical), environmental and forensic testing laboratories.
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HPLC Column Selection

Column selection is based primarily on the solubility of the analytes in solvents. HPLC analytes are typically organic molecules that vary in size, polarity, and functionality. These features govern the solubility of the analyte in solvents that range from highly polar to non-polar. Some analytes have ionic functionality. Ionic analytes often require pH or ionic strength adjustments for good chromatography.

Reversed-Phase Chromatography is the branch of HPLC that uses a hydrophobic treated silica as the stationary phase. The hydrophobic treatment is derived from a silane bonding agent. Polar solvents, including water, are typically used as the mobile phase. The analytes are separated based on their retention behavior on the stationary phase. More retentive species elute later from the stationary phase. The mobile phase concentration is adjusted to give the best chromatography.

Hilic/Normal Phase Chromatography is the branch of HPLC that uses a hydrophilic silica surface as the stationary phase. Hilic/ Normal phase chromatography separates analytes based on their affinity for polar stationary phases. The analytes are typically soluble in non-polar solvents. Many normal phases are derived from silane bonding agents. Bear silica is also used in normal phase HPLC. Adjustments in mobile phase concentration are made to give the best chromatography.


Reliasil HPLC columns are designed for high efficiency (high plate count) and general utility. The proprietary silica gel manufacturing process yields a Gaussian particle size distribution, narrow pore-size distribution, and strong resistance to high pH.

Phases: Silica, C1, C4, C8, C18, C30, NH2, CN, Phenyl, SCX, SAX, Diol, Polyamine, PFP
pH Range: 2-8.5,


Orosil HPLC columns are designed for the separation of polar, semi-polar, and nonpolar compounds at low to medium pH

Phases: C8, C18
pH Range: 1.5-9.0

Orosil Polar C18
Orosil Polar C18 columns are particularly advantageous for difficult separations involving polar and semi-polar compounds.

Phases: C18
pH Range: 1.5-9.0


Matrix C18 uses base-deactivated, ultra-high purity silica gel, densely bonded to produce an inert material ideally suited for the analysis of basic compounds. The resulting hydrophobic surface is also stable over a wide pH range (2-9) to allow for excellent method flexibility.
Matix C18 AQ- Polar analysis in high Aqueous Mobile phases. Interspersed with hydrophilic end-capping group.

Phases: C8, C18, C18AQ, NH2, Polychrome
pH Range: 2-9.0


Monitor is a top-quality phase, providing excellent stability and reproducibility, and minimal secondary interaction. Monitor is a perfect choice for reversed phase separations requiring thoroughly base-deactivated columns, and excels in challenging pharmaceutical and peptide separations. We developed Monitor expressly for the chromatography of difficult-to-separate samples.

Phases: silica, C8, C18
pH Range: 2-8

Showing 1–12 of 545 results

  • Catalog#
  • Description
Isocratic Separation of Aliphatic Alcohols on an Orosil C18-HC Column Using UV Detection File size: 311 KB Download
Isocratic Separation of Ten Cannabinoids on Orosil C18-HC, 3 μm File size: 311 KB Download
Isocratic Separation of Ten Cannabinoids on Gazelle C18, 1.7 μm File size: 308 KB Download
Analysis of Tocotrienols and Tocopherols on Orosil C18, 5 μm File size: 381 KB Download
Analysis of Tocotrienols and Tocopherols on Gazelle C18, 1.7 μm File size: 375 KB Download
Isocratic Analysis of the Alcohols Present in a Generic Hand/Spray Sanitizer File size: 468 KB Download
Isocratic Analysis of Seven Cannabinoids on Orosil C18-HC File size: 261 KB Download
Isocratic Analysis of TEN Cannabinoids on Orosil C18-HC File size: 337 KB Download
Isocratic Analysis of Cannabinoids on Orosil C18-HC File size: 267 KB Download
Separation of α- and β-Carotenes on C18-HC (High Carbon) File size: 310 KB Download
Selectivity of Orosil C18-HC File size: 254 KB Download
Mephedrone Analysis by HPLC File size: 882KB Download
Comprehensive Approaches for Cannabis Product Testing Potency and Pesticide Residue Analysis File size: 882KB Download
BSA Tryptic Digest analysis-LCRE-C18-18107 File size: 227KB Download
Peptide Mixture-LCRE-C18-18109 File size: 232KB Download
Intact Proteins-LCRE-C4-18108 File size: 314KB Download
Matrix C18-Analgesics File size: 139KB Download
Matrix C18-Azithromycin File size: 85KB Download
Matrix Polychrome-beta and dexa-methasone File size: 52KB Download
Matrix Polychrome-Carboplatin File size: 40KB Download
Matrix Polychrome-Decitabine File size: 39KB Download
Matrix-Polychrome-(3epi)-25OH VD3 File size: 71KB Download
Matrix-Polychrome-Epitestosterone-testosterone File size: 49KB Download
Matrix-Polychrome-Epitestosterone-testosterone-LCMSMS File size: 70KB Download
Matrix-Polychrome-hydrocortisone-prednisolone File size: 118KB Download
Monitor C8-Carvadilol-BP method File size: 50KB Download
Monitor C18-Analgesics File size: 141KB Download
Orosil C8- carvedilol BP method File size: 49KB Download
Orosil C18-Analgesics File size: 57KB Download
Orosil C30-Carotenes File size: 134KB Download
Orosil C30-Carotenes-2 File size: 153KB Download
Orosil ODS-Drug impurities File size: 320KB Download
Orosil ODS-Omega-3 File size: 127KB Download
Orosil Polar C18-Aromatic Amines File size: 105KB Download
Orosil silica-Orange Juice File size: 166KB Download
Orosil-C18-Tamoxifen-Pregabalin-Budesonide-Eszopiclone-Eprosartan-Stavudine-Ciprofloxacin-Atracurium File size: 190KB Download
Orosil-Phenyl-Hexyl-Vitamin E File size: 117KB Download
Presstige-C18-Sun screen reagents File size: 65KB Download
Prestige C18-Pesticides File size: 150KB Download
Prestige-C18-Mycotoxin File size: 261KB Download
Prestige-C18-Neutral Basic Polar Analytes File size: 390KB Download
Reliasil Amino-Steviol Glycosides File size: 130KB Download
Reliasil C8-carvedilol BP method File size: 51KB Download
Reliasil C18-Aromatic Amines File size: 105KB Download
Reliasil C18-Xanthines File size: 105KB Download
Reliasil File size: 202KB Download
Reliasil-Amino-xylose-galactose-glucose-mannose-arabinose File size: 65KB Download
Reliasil-C8-carvedilol File size: 327KB Download
Reliasil-C18-nicotine File size: 100KB Download
Reliasil-C18-peptides Separation File size: 318KB Download
Reliasil-ODS1-Flavanoids- File size: 153KB Download
Reliasil-phenyl-Tetrahydrocannabinol File size: 197KB Download
Oligonucleotides (Oligo2024) LCRE-C8-22001 File size: 196 KB Download