HILIC & Normal Phase Chromatography Columns for Polar Separations

Orochem Technologies provides high-performance HILIC chromatography columns and normal phase HPLC columns for the separation of polar and hydrophilic compounds. Hydrophilic interaction chromatography (HILIC) uses a polar stationary phase and aqueous-organic mobile phases to deliver excellent resolution and reproducibility. Normal phase chromatography employs a hydrophilic silica surface with non-polar mobile phases for selective separation based on analyte polarity.

What is HILIC Chromatography?

Hydrophilic Interaction Chromatography (HILIC) is a variation of normal phase chromatography that uses polar stationary phases with a high proportion of organic solvents in the mobile phase. It is especially effective for highly polar analytes such as sugars, amino acids, peptides, and polar pharmaceuticals.

Key Benefit: Enhanced retention of polar compounds
Applications: Bioanalysis, metabolomics, pharmaceutical impurity profiling

What is Normal Phase Chromatography?

Normal phase chromatography separates analytes based on their affinity for polar stationary phases. It uses non-polar mobile phases and can be implemented with bare silica or bonded polar phases derived from silane chemistry. Adjusting mobile phase composition allows fine-tuning of selectivity for complex mixtures.

HILIC vs. Normal Phase Chromatography

While both methods use polar stationary phases, HILIC chromatography typically employs aqueous-organic mobile phases and is more suited for highly polar compounds. Normal phase chromatography uses non-polar mobile phases and is often preferred for moderately polar analytes or when water is incompatible with the sample.

Orochem HILIC & Normal Phase Column Options

Our HILIC and normal phase HPLC columns are available in multiple silica chemistries, dimensions, and particle sizes to support analytical, preparative, and SMB-scale separations. Proprietary silica manufacturing ensures:

Narrow particle size distribution
Consistent pore structure
Excellent reproducibility batch-to-batch

Order HILIC & Normal Phase Columns

Explore our full range of HILIC columns and normal phase chromatography columns for precise polar separations. Contact our chromatography experts for selection guidance.