Chiral Chromatography

Orochem Technologies offers a family of polysaccharide-based chiral stationary phases (CSPs) for liquid chromatography under the Epitomize brand name. The CSPs are available in particle sizes from 1.7 microns up to 300 microns, and columns can be ordered in many different configurations for use in UHPLC, HPLC, prep/semi-preparative LC, and simulated moving bed (SMB) chromatography. The Epitomize family of chiral stationary phases is composed of two series. Epitomize CSP-1 is based on derivatized polysaccharides that are coated onto modified silica gel.

Epitomize CSP-2 is based on derivatized polysaccharides that are covalently-bound to silica gel. The best CSP for a given separation depends on several factors. For the racemate, these factors include structure, polarity, and the type and number of functional groups present. Although all of the CSPs are based on polysaccharides, each typically shows a different chiral response for the same racemate. This response can also change dramatically with the selection of mobile phase. Together, these CSPs permit the separation of a very broad range of racemates. Both the CSP-1 and CSP-2 Series can be used in normal phase, polar phase, reverse phase, and SFC. Standard modifiers are TEA, DEA, ethanolamine, TFA, acetic acid, etc. and the use level is typically around 0.1%.

Each column is tested before packaging and a chromatogram with test conditions and column performance data is included.

More Details

Epitomize CSP-1 Series

The Epitomize CSP-1 Series of chiral stationary phases is based on derivatized polysaccharides that are coated onto modified silica gel. Typical of coated CSPs, they should not be used with certain solvents such as ketones (e.g., acetone), halogenated solvents (e.g., dichloromethane and chloroform), ethyl acetate, THF, MTBE, aromatics, or other aggressive solvents such as DMF and DMA. Such solvents may diminish or destroy the effectiveness of the CSP-1 Series of chiral stationary phases. The CSP-1 Series is shown in Table 1.

CSP-1ZCellulose tris-(3-chloro-4-methylphenylcarbamate)

CSP Chiral Phase
CSP-1A Amylose tris-(3,5-dimethylphenylcarbamate)
CSP-1C Cellulose tris-(3,5-dimethylphenylcarbamate)
CSP-1J Cellulose tris-(4-methylbenzoate)

Epitomize CSP-2 Series

The Epitomize CSP-2 Series of chiral stationary phases is based on derivatized polysaccharides that are covalently-bound to silica gel. The covalent linkage permits the use of all typical organic solvents including ketones (e.g., acetone), halogenated solvents (e.g., dichloromethane and chloroform), ethyl acetate, THF, MTBE, aromatics, and other aggressive solvents such as DMF and DMA. The CSP-2 series allows the separation of many racemates that cannot be separated on coated CSPs due to solvent or mobile phase limitations. Due to the different bonding techniques used for the Epitomize CSP-1 Series and the CSP-2 Series, the user should expect some selectivity differences for some racemates. The CSP-2 Series is shown in Table 2.

CSP Chiral Phase
CSP-2A Amylose tris-(3,5-dimethylphenylcarbamate)
CSP-2C Cellulose tris-(3,5-dimethylphenylcarbamate)
CSP-2Z Cellulose tris-(3-chloro-4-methylphenylcarbamate)

Showing 1–12 of 215 results

  • Catalog#
  • Description
Chiral HPLC Column File size: 1 MB Download
CSP-1A-5 micron column-Stilbene Oxide, trans File size: 112KB Download
CSP-1A-5 micron Column-Trogers Base File size: 114KB Download
CSP-1A-5micron Column-Benzoin File size: 110KB Download
CSP-1A-UHPLC Column-Benzoin File size: 112KB Download
CSP-1A-UHPLC Column-Flavanone File size: 112KB Download
CSP-1A-UHPLC Column-Mianserin File size: 112KB Download
CSP-1A-UHPLC Column-Stilbene Oxide, trans File size: 112KB Download
CSP-1A-UHPLC Column-Trogers Base File size: 136KB Download
CSP-1C-5 micron Column-Benzoin File size: 108KB Download
CSP-1C-5 micron Column-Bi-2-Naphthol File size: 132KB Download
CSP-1C-5 micron Column-Butanediol Dibenzoate File size: 111KB Download
CSP-1C-5 micron Column-Mianserin-HCl File size: 108KB Download
CSP-1C-5 micron Column-Warfarin File size: 111KB Download
CSP-1C-5 micron-Phenoxy-2-propanol, 1-_ File size: 111KB Download
CSP-1C-UHPLC Column-Stilbene Oxide, trans File size: 110KB Download
CSP-1J-5 micron Column-Naphthyl)ethanol_1-(1) File size: 110KB Download
CSP-1J-5 micron column-Stilbene Oxide, trans File size: 110KB Download
CSP-1J-UHPLC Column-Benzoin File size: 112KB Download
CSP-1J-UHPLC Column-Naphthyl)ethanol_1-(1- File size: 114KB Download
CSP-1J-UHPLC column-Naphthyl)ethanol_1-(2- File size: 113KB Download
CSP-1J-UHPLC Column-Stilbene Oxide, trans File size: 113KB Download
CSP-1K-5 micron Column-Flavanone File size: 119KB Download
CSP-1K-5 micron Column-Mianserin File size: 119KB Download
CSP-1Z-5 micron Column-Naphthyl)ethanol_1-(1 File size: 111KB Download
CSP-1Z-5 micron column-Phenoxy-2-propanol File size: 112KB Download
CSP-1Z-5 micron Column-Stilbene Oxide, trans File size: 119KB Download
CSP-1Z-5 micron Column-Tetrahydro-1-naphthol File size: 111KB Download
CSP-2C-5 micron Column-Bi-2-Naphthol File size: 130KB Download
CSP-2C-5 micron Column-Stilbene Oxide, trans File size: 112KB Download
CSP-2C-5 micron Column-Warfarin (2) File size: 112KB Download
CSP-2C-5 micron Column-Warfarin File size: 112KB Download
SFC-1Z-3 micron- Column-Napropamid File size: 123KB Download
SFC-CSP-1A-3 micron Column-Chlorpheniramine File size: 121KB Download
SFC-CSP-1A-3 micron Column-Phenoxy-2-propanol, 1- File size: 118KB Download
SFC-CSP-1A-3 micron Column-Propranolol File size: 120KB Download
SFC-CSP-1A-3 micron Column-Uniconazole File size: 170KB Download
SFC-CSP-1Z-3 micron Column-Acenaphthenol, 1- File size: 120KB Download
SFC-CSP-1Z-3 micron Column-Proglumide File size: 124KB Download