Solid Phase Extraction

“SPE is a method of sample preparation that concentrates and purifies analytes from solution by sorption onto a sorbent contained in either a disposable solid phase cartridge or a 96-well plate, followed by elution of the analyte with a solvent and reconstitution into a mobile phase that is appropriate for the instrumental method used for analysis” (E.M.Thurman and M.S.Mills, Solid-Phase Extraction – Principles and Practice, John Wiley, New York, 1998)
More Details

General Steps of an SPE protocol:

  • Sample Pre-treatment: Dependent on compound of interest, sample matrix, and nature of retention chemistry; involves pH adjustment, centrifugation, filtration, dilution, buffer addition, etc.
  • Conditioning: Solvent is passed through the SPE material to wet the bonded functional groups => ensures consistent interaction.
  • Equilibration: Sorbent/ phase is treated with a solution that is similar (in polarity, pH, etc.) to the sample matrix => maximizes retention.
  • Sample Load: Introduction of the sample = analytes of interest are bound/ extracted onto the phase/sorbent.
  • Washing: Selectively remove unwanted interferences co-extracted with the analyte without prematurely eluting analytes of interest.
  • Elution: Removing analytes of interest with a solvent that overcomes the primary and secondary retention interactions between sorbent and analytes of interest.
  • Evaporation of eluent/ reconstitution with mobile phase (optional).

Strategies in carrying out SPE

There are 3 different elution strategies in SPE. Which one to choose depends on the goal of the extraction.

i. Bind-Elute Strategy

  • Most common
  • Bind: Analytes bind to tube, unwanted matrix components are washed off
  • Elute: Eluant changed to remove analytes from tube
  • Analytes are concentrated via evaporation prior to HPLC or GC analysis

ii. Interference Removal Strategy

  • Bind all unwanted matrix components and allow analytes to pass through during the sample loading stage
  • Like chemical filtration

iii. Fractionation Strategy

  • Retain and sequentially elute different classes of compounds by modifying eluant pH or % organic

SPE Products Selection Guide

Stationary Phase Selection Guide

spe-Product-guide

SPE Hardware Selection Guide

spe-hardware-guide

Features 

Oro-Sep SPE Cartridges

Oro-Sep Cartridges are molded from medical grade virgin polypropylene, fritted with 20 micron PE frits and are compatible with a syringe or a vacuum manifold. The female luer tip inlet of the cartridge allows connection to a syringe tip. The male luer tip outlet connects to a vacuum manifold. Sep-Pak®cartridges may be substituted with Oro-Sep cartridges. Oro-Sep cartridges packed with reversed phases, normal phases, and ion exchange phases in three different bed sizes, may be utilized for sample preparation and sample concentration in the bio-tech, clinical, environmental, forensic, and pharmaceutical arena.

SuPErscreen Disc - SPE

Orochem Technologies has now developed SuPErScreen, a proprietary high capacity silica particle embedded glass fiber for Disc Solid Phase Extraction. Elimination of sorbent fines and decreased sample elution columns provide cleaner extracts which allows you to bypass evaporation prior to detection.

SuPErScreen Silica impregnated glass fiber discs are manufactured using both long and short glass fibers offering greater porosity of the matrix. With silica particles present as an integral component during manufacturing, uniform distribution of silica is achieved across the thickness of the disc membrane. A typical 8 mm diameter OROCHEM disc in a 3 ml column contains approximately 13.8 mg of silica.

OmniSorb SPE Plates

  • Omnisorb 96 well filter plates are ideal for all SPE applications and High Throughput Screening.
  • 1ml sample volume per well Robotic and liquid handling compatibility Choice of chemistries.
  • Made from solvent resistant, low extractable polypropylene with unique drip directors.

Orochem 2000 Sorbent filled SPE Plate

Features & Benefits

OROCHEM 2000 SPE 96-well Plates are packed with Orpheus silica based sorbents that have broad selectivity and resist occlusion by proteinaceous materials from samples. Non-occlusion of sorbents, combination of size exclusion and adsorption chromatography allows efficient flow characteristics, enhanced detection, and cleaner extracts. The 2.2 mL/well SPE plates manufactured from virgin medical grade polypropylene have the SBS standard footprint. Individually fritted wells eliminate cross-talk; the larger exit diameter of the well enables a faster flow rate. The OROCHEM 2000 Vacuum Manifold is designed to accommodate every filter plate, and comes with inserts to accommodate collection plates of all sizes, including shallow well plates

Applications

Sample preparation by Solid Phase Extraction in drug development, drug analysis, pharmacokinetics, and biotechnological applications.

Orochem SuPErscreen Disc- SPE Plate

Features & Benefits

Orochem SuPErScreen Disc SPE is manufactured using both long and short glass fibers offering greater porosity of the matrix. With silica particles present as an integral component during manufacturing, uniform distribution of silica is achieved across the thickness of the disc membrane. The use of thick glass fibers offers increased contact with the phase. With SuPErScreen disc SPE, an 8mm diameter disc allows 13.8 mg and a 6mm disc in a 96-well format allows 7.6mg /well media loading capacity.

Applications

Sample preparation by Solid Phase Extraction in drug development, drug analysis, pharmacokinetics, and biotechnological applications.

Orochem 3000 384-well Disc SPE Plate

Orochem 3000 SPE plates are available with SuPErScreen glass fiber based SPE discs. The high throughput 384-well SPE plate enables a four-fold higher sample preparation capability. Orochem 384-well SPE plates offer yet another example of our capabilities to customize sample preparation.

  • Catalog#
  • Description
  • Silica based

    Silica based (427)

    Silica based Reversed Phase Cartridges

    Overview

    Orochem Silica based Orpheus sorbents are custom bonded in-house to standard or custom specifications. Most commonly they include irregular shaped about 50 micron particles with average 60 Å pore sizes for sample preparation.
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  • Polymer Based

    Polymer Based (61)

    Polymer based solid phase extraction products

    Overview

    Orochem offers three brands of poly DVB SPE products. Among them, Panthera and Celerity are reversed phases, and Agility products are ion-exchange modes.

    Panthera Deluxe Polymeric SPE Products

    Panthera Deluxe SPE products are the new offering from Orochem’s wide range of SPE products, which are high performing and affordable. Panthera Deluxe sorbent is based on a poly-DVB polymer, which provides both hydrophobic and hydrophilic retention. It has high capacity and retention of a full range of neutral, basic and acidic chemicals.  Most suitable for pain management drugs and other pharmaceuticals.

    Celerity Polymeric SPE Products

    Celerity DVB SPE products have been used globally for separation analytes from biological fluids. Excellent efficiency in removing salts and proteins from analytes. Three different types of Celerity products are offered based on analytes' polarity and hydrophilicity: Celerity Deluxe, Celerity Hydrophilic (HL) and Celerity Hydrophobic (HB)

    Agility Polymeric Ion Exchange SPE Products

    Agility DVB ion exchange SPE products are available in four different chemistries: SCX, SAX, WCX, and WAX.  Ideal for extraction of a wide range of acidic or basic compounds. Most suited for pharmaceutical, environmental applications and peptide analyses.
  • Carbograph

    Carbograph (4)

    Carbograph

    Overview

    Carbograph SPE Tubes are used for the acid, base and neutral extraction of pesticides and herbicides. It is also used to extract polar compounds from aqueous matrices, and removing colors.
  • Alumina

    Alumina (1)

    Alumina

    Overview





    Acidic- Lewis acid properties of alumina allow the sorbent to be more retentive towards electron-rich compounds. Basic- Lewis base properties of alumina allow the sorbent to be more retentive towards electron-deficient compounds. Neutral- Extremely polar sorbent prepared as an electrically neutral surface. Favors retention of aromatic compounds, aliphatic amines, and electronegative compounds.
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