Solid Phase Extraction

“SPE is a method of sample preparation that concentrates and purifies analytes from solution by sorption onto a sorbent contained in either a disposable solid phase cartridge or a 96-well plate, followed by elution of the analyte with a solvent and reconstitution into a mobile phase that is appropriate for the instrumental method used for analysis” (E.M.Thurman and M.S.Mills, Solid-Phase Extraction – Principles and Practice, John Wiley, New York, 1998)
More Details

General Steps of an SPE protocol:

  • Sample Pre-treatment: Dependent on compound of interest, sample matrix, and nature of retention chemistry; involves pH adjustment, centrifugation, filtration, dilution, buffer addition, etc.
  • Conditioning: Solvent is passed through the SPE material to wet the bonded functional groups => ensures consistent interaction.
  • Equilibration: Sorbent/ phase is treated with a solution that is similar (in polarity, pH, etc.) to the sample matrix => maximizes retention.
  • Sample Load: Introduction of the sample = analytes of interest are bound/ extracted onto the phase/sorbent.
  • Washing: Selectively remove unwanted interferences co-extracted with the analyte without prematurely eluting analytes of interest.
  • Elution: Removing analytes of interest with a solvent that overcomes the primary and secondary retention interactions between sorbent and analytes of interest.
  • Evaporation of eluent/ reconstitution with mobile phase (optional).

Strategies in carrying out SPE

There are 3 different elution strategies in SPE. Which one to choose depends on the goal of the extraction.

i. Bind-Elute Strategy

  • Most common
  • Bind: Analytes bind to tube, unwanted matrix components are washed off
  • Elute: Eluant changed to remove analytes from tube
  • Analytes are concentrated via evaporation prior to HPLC or GC analysis

ii. Interference Removal Strategy

  • Bind all unwanted matrix components and allow analytes to pass through during the sample loading stage
  • Like chemical filtration

iii. Fractionation Strategy

  • Retain and sequentially elute different classes of compounds by modifying eluant pH or % organic

SPE Products Selection Guide

Stationary Phase Selection Guide

spe-Product-guide

SPE Hardware Selection Guide

SAMPLE PREP

ORPHEUS CLASSIC SPE CARTRIDGE 1, 3, 6, 12,
20, 60 ML
Solid-Phase-Extraction
ORO-SEP CARTRIDGE WITH FEMALE & MALE LUER TIPS Solid-Phase-Extraction
LARGE RESERVOIR COLUMN (LRC) LARGE RESERVOIR ON THE TOP & REGULAR 1 OR 3 ML CARTRIDGE WITH SORBENT AT THE BOTTOM 10 ML Solid-Phase-Extraction
OROSCREEN LARGE SPE CARTRIDGE 8 ML Solid-Phase-Extraction
OMNISORB 96-WELL PLATE 1 ML/WELL Solid-Phase-Extraction
OROCHEM 2000 96-WELL PLATE 2 ML/WELL Solid-Phase-Extraction
SUPERSCREEN DISC DISC COLUMNS 6 or 8 MM DISC
96-WELL DISC PLATE 6 MM DISC
1 ML/WELL
OROCHEM 3000 384-WELL DISC PLATE Solid-Phase-Extraction

Features 

Oro-Sep SPE Cartridges

Oro-Sep Cartridges are molded from medical grade virgin polypropylene, fritted with 20 micron PE frits and are compatible with a syringe or a vacuum manifold. The female luer tip inlet of the cartridge allows connection to a syringe tip. The male luer tip outlet connects to a vacuum manifold. Sep-Pak®cartridges may be substituted with Oro-Sep cartridges. Oro-Sep cartridges packed with reversed phases, normal phases, and ion exchange phases in three different bed sizes, may be utilized for sample preparation and sample concentration in the bio-tech, clinical, environmental, forensic, and pharmaceutical arena.

SuPErscreen Disc - SPE

Orochem Technologies has now developed SuPErScreen, a proprietary high capacity silica particle embedded glass fiber for Disc Solid Phase Extraction. Elimination of sorbent fines and decreased sample elution columns provide cleaner extracts which allows you to bypass evaporation prior to detection.

SuPErScreen Silica impregnated glass fiber discs are manufactured using both long and short glass fibers offering greater porosity of the matrix. With silica particles present as an integral component during manufacturing, uniform distribution of silica is achieved across the thickness of the disc membrane. A typical 8 mm diameter OROCHEM disc in a 3 ml column contains approximately 13.8 mg of silica.

OmniSorb SPE Plates

  • Omnisorb 96 well filter plates are ideal for all SPE applications and High Throughput Screening.
  • 1ml sample volume per well Robotic and liquid handling compatibility Choice of chemistries.
  • Made from solvent resistant, low extractable polypropylene with unique drip directors.

Orochem 2000 Sorbent filled SPE Plate

Features & Benefits

OROCHEM 2000 SPE 96-well Plates are packed with Orpheus silica based sorbents that have broad selectivity and resist occlusion by proteinaceous materials from samples. Non-occlusion of sorbents, combination of size exclusion and adsorption chromatography allows efficient flow characteristics, enhanced detection, and cleaner extracts. The 2.2 mL/well SPE plates manufactured from virgin medical grade polypropylene have the SBS standard footprint. Individually fritted wells eliminate cross-talk; the larger exit diameter of the well enables a faster flow rate. The OROCHEM 2000 Vacuum Manifold is designed to accommodate every filter plate, and comes with inserts to accommodate collection plates of all sizes, including shallow well plates

Applications

Sample preparation by Solid Phase Extraction in drug development, drug analysis, pharmacokinetics, and biotechnological applications.

Orochem SuPErscreen Disc- SPE Plate

Features & Benefits

Orochem SuPErScreen Disc SPE is manufactured using both long and short glass fibers offering greater porosity of the matrix. With silica particles present as an integral component during manufacturing, uniform distribution of silica is achieved across the thickness of the disc membrane. The use of thick glass fibers offers increased contact with the phase. With SuPErScreen disc SPE, an 8mm diameter disc allows 13.8 mg and a 6mm disc in a 96-well format allows 7.6mg /well media loading capacity.

Applications

Sample preparation by Solid Phase Extraction in drug development, drug analysis, pharmacokinetics, and biotechnological applications.

Orochem 3000 384-well Disc SPE Plate

Orochem 3000 SPE plates are available with SuPErScreen glass fiber based SPE discs. The high throughput 384-well SPE plate enables a four-fold higher sample preparation capability. Orochem 384-well SPE plates offer yet another example of our capabilities to customize sample preparation.

  • Catalog#
  • Description
  • Silica based

    Silica based (427)

    Silica-based Reversed Phase Cartridges

    Overview

    Orochem Silica based Orpheus sorbents are custom bonded in-house to standard or custom specifications. Most commonly they include irregular shaped about 50 micron particles with average 60 Ă… pore sizes for sample preparation.
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  • Polymer Based

    Polymer Based (29)

    Polymer based solid phase extraction products

    Orochem synthesizes a full range of polymer-based, solid phase extraction (SPE) sorbents. Every sorbent batch must pass a series of quality control tests to ensure batch-to-batch reproducibility and performance. Orochem’s Panthera Deluxe and Agility Deluxe products provide high performance at an affordable price. Sorbents are available in cartridges, 96-well plates, and microelute plate format. Combined method development 96-well plates can be custom made upon request. Polymer Based

    Panthera Deluxe Polymeric SPE Products

    The Panthera Deluxe sorbent is based on poly-DVB and provides both hydrophobic and hydrophilic retention. It demonstrates high capacity and retention for a full range of neutral, basic, and acidic compounds and is particularly suitable for pain management drugs and other pharmaceuticals.

    Technical Specifications:

    • Mode: Reversed phase
    • Average Particle Size: 15 µm
    • Average Pore Size: 39 Ă…
    • Sorbent Substrate: Polymeric DVB
    • Water Wettable: Yes
    • Mass Spec Compatible: Yes
    • pH Range: 0-14

    Panthera Customer Applications

    Orochem customers use Panthera to separate the following molecules:
    • Reloxifene
    • Albendazole
    • Topiramate
    • Pregabalin
    • Dolutegravir
    • Dapagliflozine
    • Ibuprofen
    • Loratidine
    • Bile Acids
    • Paliperidon
    • Sofosbeuvir
    • Chlorozoxazone
    • Pramipexole
    • Hydroxychloroquine
    • Isosorbide Mononitrate
    • BUPIVACAINE Liposomal
    • Amphotericine B Liposomal

    NEW AGILITY DELUXE POLYMERIC ION-EXCHANGE SPE PRODUCTS

    Agility Deluxe poly-DVB-based ion-exchange SPE products are available in four different chemistries: SCX, SAX, WCX, and WAX. These sorbents are ideal for extraction of a wide range of acidic or basic compounds and are particularly suited for pharmaceutical, environmental applications, and peptide analyses. Agility Deluxe SCX is a mixed mode sorbent that combines strong cation exchange with a reversed phase. It allows neutral and acid compound elution with methanol and basic compound elution with base in an organic solvent. This sorbent targets weakly basic compounds.

    Technical Specifications:

    • Mode: Mixed mode, strong cation-exchange and reversed phase
    • Ion Exchange Capacity (Sulfonic acid content): 0.8-1.2 meq/gram
    • Functional Group: SO3-
    • Average Particle Size: 15 µm
    • Average Pore Size: 39 Ă…
    • Sorbent Substrate: Polymeric DVB
    • Water Wettable: Yes
    • Mass Spec Compatible: Yes
    • pH Range: 0-14
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  • Carbograph

    Carbograph (4)

    Carbograph

    Overview

    Carbograph SPE Tubes are used for the acid, base and neutral extraction of pesticides and herbicides. It is also used to extract polar compounds from aqueous matrices, and removing colors.
  • Gel Free SPE Cartridge

    Gel Free SPE Cartridge (1)

    Gel Free SPE Cartridge

    Do you need a quick sample preparation method for gel and cream products? Orochem offers new Gel Free SPE cartridges to remove carbomer from gel and cream products. There are two types: Gel Free LC SPE cartridges for sample preparation before injection to HPLC; while Gel Free GC SPE cartridges for GC samples.  It is a one-step SPE method and no method development is required.  We have developed and validated organic impurities in hand sanitizer gel testing methods using Gel Free GC SPE cartridges.

    Compounds:

    acetaldehyde, acetone, ethyl acetate, diethyl acetal, methanol, benzene, 2-butanol,n-propanol, iso-butanol, n-butanol, iso-amylalcohol, n-amyl alcohol
    Gel Free SPE Cartridge

    SPE EXTRACTION METHOD:

    Gel Free SPE Cartridge

    GC-FID METHOD

    Column: Agilent DB-FATWAX, 30 m, 0.25 mm diameter, 0.25 µm film Inlet Temp: 250°C Detector Temp: 250 °C
    Oven Temp Gradient Table

    Rate (°C/min) Value (°C) Hold Time (min)
    Initial
    32 10
    Ramp1 10 100 0
    Ramp2 5 220 5
    Column flow rate: 0.7 mL/min

    Injection volume: 1 pL

    Run time: 29.8 min

    Gel Free SPE Cartridge
    Gel Free SPE Cartridge
    Analyte Retention Time (min)
    Acetaldehyde 3.24
    Acetone 4.42
    Ethylacetate 6.07
    Diethyl acetal 6.20
    Methanol 6.48
    Benzene 7.85
    2-Butanol 12.70
    n-Propanol 13.05
    Iso-Butanol 14.58
    n-Butanol 15.83
    Iso-Amyl alcohol 17.18
    n-Amyl alcohol 17.91
    Analyte Linear range (ppm)
    Acetaldehyde 25-500
    Acetone 30-2000
    Ethylacetate 25-1000
    Diethyl acetal 25 -1000
    Methanol 25 -1000
    Benzene 2-80
    2-Butanol 25-1000
    n-Propanol 25-1000
    Iso-Butanol 30-2000
    n-Butanol 25-1000
    Iso-Amyl alcohol 30-2000
    n-Amyl alcohol 25-1000
AQUAMATRIX SLLE File size: 376KB Download
RUBYPRO Protein Crash File size: 846KB Download
Orpheus File size: 3.97MB Download
Panthera Deluxe SPE Cartridge File size: 2.7MB Download